Alkaline Phosphatase,
Calf Intestinal (CIAP)

#3002

 

1,000 units

#3002L

 

5,000 units

DESCRIPTION
Alkaline Phosphatase catalyszes the removal of 5'-phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates.

SOURCE
Calf intestinal mucosa.

APPLICATIONS
Removing 5' phosphates from DNA or RNA prior to end labeling.
Removing 5' phosphates from fragments of DNA to prevent self-ligation.

UNIT DEFINITION
One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1mol of 4-nitrophenyl- phosphate to 4-nitrophenol in 1 minute at 37.

STANDARD UNIT ASSAY CONDITIONS
1M diethanolamine (pH9.8), 0.5mM MgCl2, 10.9mM 4-nitrophenylphosphate.

CONCENTRATION: 1-5 unit/l.

10X REACTION BUFFER
500mM Tris-HCl (pH9.3), 10mM MgCl2, 1mM ZnCl2, 10mM spermidine.

STORAGE CONDITIONS
10mM Tris-HCl (pH8.0), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl and 50% glycerol. Store at -20.

REFERENCE
1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, second ed., Cold Spring Harbor Laboratory, Cold Spring Harbor.