Mung Bean Nuclease

#3006

 

1,500 units

#3006L

 

7,500 units

DESCRIPTION
Mung Bean Nuclease degrades single-stranded nucleic acids to yield 5'-phosphoryl mono- or oligonucleotides. Double-stranded DNA, RNA and DNA:RNA hybrids are relatively resistant to the enzyme. If very large amount of enzyme are used it also degrades double-stranded DNA from both ends. Unlike S1 nuclease, this enzyme will not cleave the DNA strand opposite a nick in a duplex.

SOURCE
Mung bean sprouts.

APPLICATIONS
Removal of 3' and 5' extensions from DNA or RNA termini.
Transcript mapping.
Opening up hairpin loops formed during synthesis of double-stranded cDNA.
Excision of gene coding sequences from genomic DNA.

UNIT DEFINITION
One unit is defined as the amount of enzyme required to produce 1g of acid-soluble nucleotides 1 minutes at 37.

STANDARD UNIT ASSAY CONDITIONS
30mM sodium acetate (pH4.6), 50mM NaCl, 1mM ZnCl2, 0.5mg/ml denatured calf thymus DNA and 5%(v/v) glycerol.

CONCENTRATION: 50-100 units/ul.

10X REACTION BUFFER
300mM sodium acetate(pH5.0), 500mM NaCl, 10mM ZnCl2.

STORAGE CONDITIONS
10mM sodium acetate (pH5.0), 0.1mM zinc acetate, 1mM cysteine, 0.001% Triton X-100, and 50% glycerol. Store at -20.

REFERENCES
1. Kowalski, D. et al. (1976) Biochemistry 15, 4457.
2. Green, M.R. and Roeder, R.G. (1980) Cell 22, 231.
3. Gubler, U. (1987) Methods Enzymol. 152, 330.