Taq DNA polymerase is heat
stable DNA polymerase purified from thermophilic bacterium Thermus
aquaticus. This enzyme has an optimum temperature around 74¡É and can
withstand temperatures up to 95¡É. The enzyme is unmodified and has a
molecular weight of 94kDa (SDS-PAGE). Our Taq DNA polymerase is provided
in two different storage buffers.
Thermus aquaticus strain
One unit is
defined as the amount of enzyme required to catalyze the incorporation of
10nmol of dNTPs into an acid-insoluble product in 30 minutes at
Patent No. 4,889,818, with respect to a purified thermostable DNA
polymerase isolated from Thermus aquaticus,> has been granted to
Hoffmann-La Roche. This product has not been licensed for use in the
polymerase chain reaction (PCR) process for amplifying nucleic acid
covered by U.S.Patent Nos. 4,683,195 and 4,683,202 owned by Hoffmann-La
Roche. Patents pending or issued in other countries.
STANDARD UNIT ASSAY CONDITIONS
Tris-HCl (pH9.0 at 25¡É), 50mM NaCl, 10mM MgCl2, 200¥ìM dATP, dCTP, dGTP, and
radiolabeled dTTP, and 12.5¥ìg activated calf thymus DNA in a 50¥ìl
CONCENTRATION: 5 units/¥ìl.
10X REACTION BUFFER w/o
(pH8.3 at 25¡É), 500mM KCl. Buffer is optimized for use with 0.2mM for each
10X REACTION BUFFER w/15mM
100mM Tris-HCl (pH8.3
at 25¡É), 500mM KCl, and 15mM MgCl2. Buffer is optimized for use
with 0.2mM for each of dNTPs.
MAGNESIUM CHLORIDE: 25mM
(pH8.0), 100mM NaCl, 0.1mM EDTA, 5mM DTT, 1.0% Triton X-100 and 50%
glycerol. Store at -20¡É.
1. Chien, A. et al. (1976)
J. Bacteriol. 127, 1550.
2. Kaledin, A.S. et al. (1980)
Biokhimiya 45, 494.