DESCRIPTION
Taq DNA polymerase is heat stable DNA polymerase purified from thermophilic bacterium Thermus aquaticus.  This enzyme has an optimum temperature around 74℃ and can withstand temperatures up to 95℃.  The enzyme is unmodified and has a molecular weight of 94kDa (SDS-PAGE). Our Taq DNA polymerase is provided in two different storage buffers. 

SOURCE 
Thermus aquaticus strain YT1. 

UNIT DEFINITION 
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTPs into an acid-insoluble product in 30 minutes at 74℃. 

^*U.S. Patent No. 4,889,818, with respect to a purified thermostable DNA polymerase isolated from Thermus aquaticus,> has been granted to Hoffmann-La Roche.  This product has not been licensed for use in the polymerase chain reaction (PCR) process for amplifying nucleic acid covered by U.S.Patent Nos. 4,683,195 and 4,683,202 owned by Hoffmann-La Roche. Patents pending or issued in other countries. 

STANDARD UNIT ASSAY CONDITIONS
50mM Tris-HCl (pH9.0 at 25℃), 50mM NaCl, 10mM MgCl₂, 200μM dATP, dCTP, dGTP, and radiolabeled dTTP, and 12.5μg activated calf thymus DNA in a 50μl reaction. 

CONCENTRATION: 5 units/μl. 

10X REACTION BUFFER w/o MgCl₂ 
100mM Tris-HCl (pH8.3 at 25℃), 500mM KCl. Buffer is optimized for use with 0.2mM for each of dNTPs. 

10X REACTION BUFFER w/15mM MgCl₂  
100mM Tris-HCl (pH8.3 at 25℃), 500mM KCl, and 15mM MgCl₂. Buffer is optimized for use with 0.2mM for each of dNTPs. 

MAGNESIUM CHLORIDE: 25mM MgCl₂. 

STORAGE CONDITIONS 
50mM Tris-HCl (pH8.0), 100mM NaCl, 0.1mM EDTA, 5mM DTT, 1.0% Triton X-100 and 50% glycerol.  Store at -20℃. 

REFERENCES
1. Chien, A. et al. (1976) J. Bacteriol. 127, 1550. 
2. Kaledin, A.S.  et al. (1980) Biokhimiya 45, 494.